{"id":84,"date":"2017-04-19T15:52:12","date_gmt":"2017-04-19T15:52:12","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/csb\/?page_id=84"},"modified":"2023-12-02T18:25:21","modified_gmt":"2023-12-03T00:25:21","slug":"expression-vectors","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/csb\/facilities\/labs-instrumentation-facility\/protein-characterization\/expression-vectors\/","title":{"rendered":"Expression Vectors"},"content":{"rendered":"

CSB plasmids are freely available to 91ºÚÁÏÍø researchers. Contact Noah Saracho<\/a><\/span><\/p>\n

for more information. The links for ‘Single Gene Expression’ require login with VUNet ID and password.<\/span><\/p>\n

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Single Gene Expression in E. coli<\/i>:<\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
NT Tag(s)<\/th>\nProtease Cleavage Site<\/th>\nCT Tag(s)<\/th>\nResistance<\/th>\nVector<\/th>\n<\/tr>\n
GST<\/td>\nTEV<\/td>\nHis8<\/td>\nKan<\/td>\npAT110<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHis8<\/td>\nKan<\/td>\npAT109<\/a><\/td>\n<\/tr>\n
His6<\/td>\nThrombin<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npSV277<\/a><\/td>\n<\/tr>\n
TEV<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npSV281<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npBG100<\/a><\/td>\n<\/tr>\n
His6<\/td>\nAmp<\/td>\npHD116<\/a><\/td>\n<\/tr>\n
His10<\/td>\nThrombin<\/td>\nHis8<\/td>\nKan<\/td>\npNR114<\/a><\/td>\n<\/tr>\n
TEV<\/td>\nHis8<\/td>\nKan<\/td>\npNR111<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHis8<\/td>\nKan<\/td>\npBG106<\/a><\/td>\n<\/tr>\n
His\u2022GST<\/td>\nTEV<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npBG105<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npBG101<\/a><\/td>\n<\/tr>\n
His\u2022MBP<\/td>\nThrombin<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npSV278<\/a><\/td>\n<\/tr>\n
TEV<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npSV282<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npLM302<\/a>
\npLM303<\/a><\/td>\n<\/tr>\n
His\u2022SUMO<\/td>\nTEV<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npHD115<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npBG102<\/a>
\npBG103<\/a><\/td>\n<\/tr>\n
Ulp1<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npBG104<\/a><\/td>\n<\/tr>\n
MBP<\/td>\nTEV<\/td>\nHis8<\/td>\nKan<\/td>\npAT108<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHis8<\/td>\nKan<\/td>\npAT107<\/a><\/td>\n<\/tr>\n
Trx\u2022His<\/td>\nTEV<\/td>\nHis6<\/td>\nAmp<\/td>\npNR113<\/a><\/td>\n<\/tr>\n
3C\/PreScission<\/td>\nHSV\u2022His6<\/td>\nKan<\/td>\npLM304<\/a><\/td>\n<\/tr>\n
His6<\/td>\nAmp<\/td>\npNR112<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/span><\/p>\n

Multiple Gene Expression:<\/span><\/h3>\n